publication . Other literature type . 2019

Additional file 9: of Peripheral blood DNA methylation differences in twin pairs discordant for Alzheimer’s disease

Konki, Mikko; Malonzo, Maia; Karlsson, Ida; Lindgren, Noora; Bishwa Ghimire; Smolander, Johannes; Scheinin, Noora; Ollikainen, Miina; Laiho, Asta; Elo, Laura; ...
Open Access
  • Published: 01 Jan 2019
  • Publisher: figshare
Abstract
DNA methylation level of the ADARB2 mark is higher in the Alzheimer’s disease cases with a history of smoking. Smoking history was included in the linear mixed effects model as a fixed effect, and interaction term, to determine whether this adjustment attenuates association of the ADARB2 DNA methylation mark with Alzheimer’s disease. The analysis was carried out by including all the 60 twin pairs with smoking data available. According to the results the disease association remained significant also when smoking was included in the model (estimate 64.51%, SE 9.23, Wald t value 6.99, not adjusted for APOE genotype). Smoking did interact with the disease status (estimate 8.10%, SE 1.43, Wald t value 5.68) and the median methylation level was increased in the cases with a smoking history in comparison to non-smoking cases and controls as well as controls with a smoking history. The association remained significant also when APOE genotype was included in the model (estimate 35.41%, SE 9.41 and Wald t value 3.76). A) DNA methylation levels for the ADARB2 mark in non-smokers and past or current smokers are shown separately for females with APOE ε33 genotype who differ from the other groups and show no disease association. B) Residual plot for the full model: me ~ dis + dis * zyg + dis * age + dis * sex + dis * smo + dis * apoe + (1 | pairid/pos). me = CpG methylation level, dis = disease status, zyg = zygosity, smo = smoking history (never, ever), apoe = apoe genotype group, pairid = twin pair information, pos = genomic position. (PDF 715 kb)
Funded by
AKA| Epigenetic pathways to obesity
Project
  • Funder: Academy of Finland (AKA)
  • Project Code: 297908
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Open Access
figshare
Other literature type . 2019
Providers: Datacite
Open Access
figshare
Other literature type . 2019
Providers: Datacite
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