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AbstractThe field of ancient DNA is taxonomically dominated by studies focusing on mammals. This taxonomic bias limits our understanding of endogenous DNA preservation for vertebrate taxa with different bone physiology, such as teleost fish. In contrast to most mammalian bone, teleost bone is typically brittle, porous, lightweight and is characterized by a lack of bone remodeling during growth. Using high-throughput shotgun sequencing, we here investigate the preservation of DNA in a range of different bone elements from over 200 archaeological Atlantic cod (Gadus morhua) specimens from 38 sites in northern Europe, dating up to 8000 years before present. We observe that the majority of archaeological sites (79%) yield endogenous DNA, with 40% of sites providing samples that contain high levels (> 20%). Library preparation success and levels of endogenous DNA depend mainly on excavation site and pre-extraction laboratory treatment. The use of pre-extraction treatments lowers the rate of library success, although — if successful — the fraction of endogenous DNA can be improved by several orders of magnitude. This trade-off between library preparation success and levels of endogenous DNA allows for alternative extraction strategies depending on the requirements of down-stream analyses and research questions. Finally, we find that — in contrast to mammalian bones — different fish bone elements yield similar levels of endogenous DNA. Our results highlight the overall suitability of archaeological fish bone as a source for ancient DNA and provide novel evidence for a possible role of bone remodeling in the preservation of endogenous DNA across different classes of vertebrates.

The Periplus of the Erythraean Sea is a Roman period guide to trade and navigation in the Indian Ocean. Justly famous for offering a contemporary and descriptive account of early Indian Ocean trade, the work has been subject to and a point of departure for numerous studies. Its extensive influence on scholarship is, however, also problematic, as it reflects the limited information and cultural and personal bias of its unknown author. Arguably this might have led scholars to overemphasise so-called western or Roman participation in early Indian Ocean trade. Network analysis allows us to map, visualize and measure interconnectedness in the Periplus Maris Erythraei. Many of these connections are not explicitly mentioned in the text, but by connecting not only places with places, but also products with places that export and import them, we get a partly different impression of Indian Ocean trade from that conventionally gathered from the Periplus. It allows us to ask questions about the relationship between coastal cabotage and transoceanic shipping, to identify regional trading circuits, and unexpected centres of long-distance exchange. publishedVersion