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description Publicationkeyboard_double_arrow_right Preprint 2023Publisher:Cold Spring Harbor Laboratory Publicly fundedFunded by:SFI | Next Generation Antibioti..., SFI | Pre-clinical testing of n..., EC | INMARE +1 projectsSFI| Next Generation Antibiotics: anti-biofilm, anti-pathogenic natural bioactives from marine microorganisms. ,SFI| Pre-clinical testing of novel fungal biofilm blockers for the medical device sector. ,EC| INMARE ,SFI| Small molecule inhibitors of HIF-1: a new class of anti-cancer therapeutics.Authors: Jose A. Caparrós-Martín; Montserrat Saladié; S. Patricia Agudelo-Romero; Kristy S. Nichol; +6 AuthorsJose A. Caparrós-Martín; Montserrat Saladié; S. Patricia Agudelo-Romero; Kristy S. Nichol; F. Jerry Reen; Yuben Moodley; Siobhain Mulrennan; Stephen M. Stick; Peter A Wark; Fergal O’Gara;AbstractBackgroundChronic obstructive pulmonary disease (COPD) is a complex disorder with a high degree of interindividual variability. Gastrointestinal dysfunction is common in COPD patients and has been proposed to influence the clinical progression of the disease. Using the presence of bile acid(s) (BA) in bronchoalveolar lavage fluid (BAL) as a marker of gastric aspiration, we evaluated the relationships between BAs, clinical outcomes, and bacterial lung colonisation.MethodsWe used BAL specimens from a cohort of COPD patients and healthy controls. Bile acids were profiled and quantified in BAL supernatants using mass spectrometry. Microbial DNA was extracted from BAL cell pellets and quantified using qPCR. We profiled the BAL microbiota using an amplicon sequencing approach targeting the V3-V4 region of the 16S rRNA gene.ResultsDetection of BAs in BAL was more likely at earliest clinical stages of COPD and was independent of the degree of airway obstruction. BAL specimens with BAs demonstrated higher bacterial biomass and lower diversity. Likewise, the odds of recovering bacterial cultures from BAL were higher if BAs were also detected. Detection of BAs in BAL was not associated with either inflammatory markers or clinical outcomes. We also observed different bacterial community types in BAL, which were associated with different clinical groups, levels of inflammatory markers, and the degree of airway obstruction.ConclusionDetection of BAs in BAL was associated with different parameters of airway ecology. Further studies are needed to evaluate whether BAs in BAL can be used to stratify patients and for predicting disease progression trajectories.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eu0 citations 0 popularity Average influence Average impulse Average Powered by BIP!more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1101/2023.06.04.23290702&type=result"></script>'); --> </script>
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description Publicationkeyboard_double_arrow_right Preprint 2023Publisher:Cold Spring Harbor Laboratory Publicly fundedFunded by:SFI | Next Generation Antibioti..., SFI | Pre-clinical testing of n..., EC | INMARE +1 projectsSFI| Next Generation Antibiotics: anti-biofilm, anti-pathogenic natural bioactives from marine microorganisms. ,SFI| Pre-clinical testing of novel fungal biofilm blockers for the medical device sector. ,EC| INMARE ,SFI| Small molecule inhibitors of HIF-1: a new class of anti-cancer therapeutics.Authors: Jose A. Caparrós-Martín; Montserrat Saladié; S. Patricia Agudelo-Romero; Kristy S. Nichol; +6 AuthorsJose A. Caparrós-Martín; Montserrat Saladié; S. Patricia Agudelo-Romero; Kristy S. Nichol; F. Jerry Reen; Yuben Moodley; Siobhain Mulrennan; Stephen M. Stick; Peter A Wark; Fergal O’Gara;AbstractBackgroundChronic obstructive pulmonary disease (COPD) is a complex disorder with a high degree of interindividual variability. Gastrointestinal dysfunction is common in COPD patients and has been proposed to influence the clinical progression of the disease. Using the presence of bile acid(s) (BA) in bronchoalveolar lavage fluid (BAL) as a marker of gastric aspiration, we evaluated the relationships between BAs, clinical outcomes, and bacterial lung colonisation.MethodsWe used BAL specimens from a cohort of COPD patients and healthy controls. Bile acids were profiled and quantified in BAL supernatants using mass spectrometry. Microbial DNA was extracted from BAL cell pellets and quantified using qPCR. We profiled the BAL microbiota using an amplicon sequencing approach targeting the V3-V4 region of the 16S rRNA gene.ResultsDetection of BAs in BAL was more likely at earliest clinical stages of COPD and was independent of the degree of airway obstruction. BAL specimens with BAs demonstrated higher bacterial biomass and lower diversity. Likewise, the odds of recovering bacterial cultures from BAL were higher if BAs were also detected. Detection of BAs in BAL was not associated with either inflammatory markers or clinical outcomes. We also observed different bacterial community types in BAL, which were associated with different clinical groups, levels of inflammatory markers, and the degree of airway obstruction.ConclusionDetection of BAs in BAL was associated with different parameters of airway ecology. Further studies are needed to evaluate whether BAs in BAL can be used to stratify patients and for predicting disease progression trajectories.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1101/2023.06.04.23290702&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu0 citations 0 popularity Average influence Average impulse Average Powered by BIP!more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1101/2023.06.04.23290702&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu