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The present study seeks to better understand the coupling of social and biophysical systems during the late Pleistocene and Holocene, a period characterized by changing interglacial conditions as well as human population expansion and intensified ecosystem management. The approach consists of a combination of patch-based archaeological survey methods, sediment column sampling for paleoenvironmental data, geospatial analysis, and machine learning for chronological unmixing, allowing the systematic evaluation of the distribution and intensity of prehistoric land use in the study area of eastern Mediterranean Iberia. Occupational and Land Use Intensity maps developed from continuous distributions of surface artifacts as well as a summed probability density curve developed from 14C dates indicate low but steady human presence in the study area during the Middle Paleolithic and Upper Paleolithic with a marked decrease of human presence across the Pleistocene/Holocene boundary. The Early (and Middle) Neolithic saw the most ubiquitous and intensive occupation of the study area followed by a significant decline during the Late Neolithic/Chalcolithic. The charcoal analysis also supports this pattern. Early Neolithic farming strategies may not have been damaging initially during the climatic regime of the Early Holocene but exacerbated the impacts of higher temperatures and summer droughts, with a loss of the most productive farmland, seen at the onset of the Late Neolithic. Similar boom-bust population trends have been documented throughout Europe during these same time spans and may indicate a recursive interaction or “coupling” between global and regional climate events and human land use strategies.

The family Rhinocerotidae is a key component of the Cenozoic European fauna. It reached its peak diversity during Miocene and began to decline in the Pliocene; the last representatives became extinct in Europe during the Quaternary. The present state-of-the-art review takes stock of the knowledge accumulated on Central and Eastern European Pleistocene rhinos to date. Eight species were present in Central and Eastern Europe in the Quaternary; five belonged to the genus Stephanorhinus (S. etruscus, S. jeanvireti, S. hundsheimensis, S. hemitoechus, and S. kirchbergensis), one was the woolly rhino Coelodonta antiquitatis, and two species were members of the genus Elasmotherium (E. peii and E. caucasicum). This review also offered the opportunity to critically reconsider some aspects of the systematics and stratigraphic location of the finds. Coelodonta antiquitatis is shown to have still been present as late as the Interplenivistulian and the Last Glacial Maximum; radiocarbon dating of its latest remains provided the timing of the final disappearance of the woolly rhino from the studied region. The Late Pleistocene distribution of Coelodonta antiquitatis in Central and Eastern Europe was found to largely overlap that of the woolly mammoth.

In the Shanshenmiaozui (SSMZ) fauna from Nihewan Basin, Canis chihliensis, Nyctereutes sp., Homotherium sp., Acinonyx sp., Lynx shansius and Pachycrocuta licenti have been identified. C. chihliensis is the dominant and best represented species whose fossil materials include crania, mandibles and postcranials, including nearly complete manus and pes. The giant cheetah Acinonyx sp. is only represented by postcranial skeletons (scapula, radius, proximal ulna and an almost complete manus), which represent the richest collection for its kind ever known in China. Nyctereutes sp. is only represented by a partial cranium and distal part of a humerus. Homotherium sp. is only represented by a partial lower m1. Lynx shansius is represented by a nearly complete mandible with p3 and m1 preserved in situ. The fossil materials of Pachycrocuta licenti include a juvenile mandible with dp2–4 and m1 attached, an isolated dp4 and a partial ascending ramus. The SSMZ carnivoran guild resembles those of the classic Nihewan fauna (CNF) and the Dmanisi fauna, which means they probably have a similar geologic age or slightly younger of the SSMZ fauna; and they also share the same open grassland/shrubland habitat. The postcranial bones of C. chihliensis and Acinonyx sp. from SSMZ represent the first records for their kinds in China.

Archaeological remains of brachyurans (e.g. crabs) are often overlooked as potential paleoenvironmental and paleoclimatical proxies in contrast to other marine and terrestrial invertebrates such as mollusks and landsnails. The potential for fine-scale regional paleoclimate reconstruction based upon these organisms' behavioral ecology has yet to be examined. We present novel isotopic analyses of the remains of a semiterrestrial freshwater crab (Potamon potamios) endemic to southwest Asia recovered from the archaeological site of Khirbat al-Mudayna al-’Aliya (KMA). KMA lies on a southern tributary of the Wadi al-Mujib in the Hashemite Kingdom of Jordan, approximately 40 km east of the Dead Sea. Excavations here recovered architecture, artifacts, and ecofacts dating to a single-period occupation during the early Iron Age (∼1000 cal BCE). Oxygen and carbon isotopes from these brachyuran remains were analyzed in conjunction with a two-year isotopic and morphometric study of the modern potomonautid population near the archaeological site and at nearby wadi systems to assess whether the isotopic values of modern crab populations correlated with variables such as temperature, including isotopic study of the pools in which crabs were found. In turn, these data could be used to reconstruct paleoclimate or paleoenvironment in the archaeological population. The high correlation in oxygen isotope values between crab carapaces and the water of the pools in which they were sampled suggest that variability in isotopic values of crab specimens recovered from KMA is tracking ancient human capture of these organisms across potentially different pools. Further, intra-year variability in isotopic values in modern crab carapaces as well as the similarity of isotopic values across wadi systems with different environments suggest that P. potamios remains cannot be used as reliable paleoenvironmental indicators. The implications of this research will have significance for archaeologists and other researchers in the study of paleoenvironment and paleoecology, and bears upon human mobility, resource acquisition, and human-nonhuman animal relationships.

International audience; While the emergence of the Acheulean is well documented in East Africa at ~1.7 Ma, subsequent developments are less well understood and to some extent controversial. Here, we provide robust evidence regarding the time period between 1.6 Ma and 1.2 Ma, based on an interdisciplinary approach to the stratigraphic sequences exposed in the Gombore gully of Melka Kunture, in the upper Awash Valley of Ethiopia. Throughout the Pleistocene, the environment differed significantly from elsewhere in Africa because of the elevation at 2000 m asl, the cooler and rainy climate, the Afromontane vegetation, the development of endemic animal species, and the recurrent impact of volcanic activity. At Gombore IB, dated ~1.6 Ma, remains of Homo erectus/ergaster have been discovered, associated with a rich early Acheulean assemblage. The techno-typological analysis of the lithic record from Gombore Iγ (~1.4 Ma) and Gombore Iδ (~1.3 Ma), where substantial areas have been excavated, and the contrasting evidence from Gombore OAM Test Pit C (~1.2 Ma), suggest that the scarcity or lack of large flakes and large cutting tools at the two earlier sites is possibly not just the outcome of sampling bias, but rather of the adaptation of H. erectus/ergaster to the local resources, in a relatively isolated environment. The sites of Gombore gully provide new evidence on the complex pattern of human evolution and adaptation in East Africa during the Lower Pleistocene.

Abstract This research aims to define a chemical, mineralogical and strontium isotope database for clayey materials collected along the Nile River banks (White Nile, Blue Nile, Atbara and Main Nile Rivers) in Sudan, to be use for prehistoric and historic pottery provenance studies. The approach here adopted consists in using the clayey materials tout court, without any pre-treatment, such as the depuration from the sand- and silt-sized fractions, in order to maintain unaltered the mineralogical and geochemical features of the natural resources possibly available also in ancient times for the pottery production, thus avoiding any possible preparation bias. Results indicate that sediments along different sections of the Nile River display characteristic geochemical and strontium isotopic signature, representing an important discrimination tool applied to ancient ceramics provenance and human mobility studies.

1. Introduction 2. Materials and methods 3. Results 3.1. Effects of low read counts on damage patterns 3.2. Identification of dietary reads from synthetic datasets 3.3. Identification of dietary reads from real datasets 4. Discussion and conclusions 4.1. Checklist for authors and reviewers 4.1.1. Field/storage contamination 4.1.2. Is the laboratory methodology suitable for aDNA authentication? 4.1.3. Is the database and pipeline suitable? 4.1.4. Is the taxonomic resolution analyzed sufficient for reliable interpretation? 4.1.5. Have unexpected taxa been reported and evaluated? 4.1.6. Have laboratory controls been evaluated alongside samples? 4.1.7. Has evidence of aDNA authenticity been provided? 4.1.8. Is there other evidence supporting that dietary organisms were consumed? Dental calculus and other preserved microbiome substrates are an attractive target for dietary reconstruction in past populations through a variety of physical, chemical, and molecular means. Recently, studies have attempted to reconstruct diet from archaeological dental calculus using archaeogenetic techniques. While dental calculus may provide a relatively stable environment for DNA preservation, the detection of plants and animals possibly consumed by an individual through DNA analysis is primarily hindered by microbial richness and incomplete reference databases. Moreover, high genomic similarity within eukaryotic groups

International audience; In southern France, the analysis of fossil dung layers from caves and shelters occupied by the first Neolithicfarmers has provided a wealth of information about the lives of shepherds and their flocks, and thus on pastoralsystems. Since the early 1980s, the development of sedimentological, archaeozoological and archaeobotanicalstudies has made possible to collect a large amount of data. More recently, the implementation of a whole rangeof innovative approaches allows a more detailed approach to pastoralism. This paper proposes a syntheticapproach of 40 years of bioarchaeological analysis on Neolithic sheepfold caves (grottes-bergeries). Their interpretation focuses on understanding the early agropastoral system: pastoral use of wild and cultivated plant resources (fodder, litter, care and health of livestock), mobility systems, seasonality, practices and appropriation ofterritory.