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description Publicationkeyboard_double_arrow_right Article 2016 FinlandPublisher:Springer Science and Business Media LLC Funded by:NSERC, NSF | Beyond Drosophila: Compar..., NSF | I-Corps: Anchored Hybrid ...NSERC ,NSF| Beyond Drosophila: Comparative transcriptomics of schizophoran flies to resolve a rapid radiation ,NSF| I-Corps: Anchored Hybrid EnrichmentAndrew D. Young; Alan R. Lemmon; Jeffrey H. Skevington; Ximo Mengual; Gunilla Ståhls; Menno Reemer; Kurt Jordaens; Scott Kelso; Emily Moriarty Lemmon; Martin Hauser; Marc De Meyer; Bernhard Misof; Brian M. Wiegmann;Background: Anchored hybrid enrichment is a form of next-generation sequencing that uses oligonucleotide probes to target conserved regions of the genome flanked by less conserved regions in order to acquire data useful for phylogenetic inference from a broad range of taxa. Once a probe kit is developed, anchored hybrid enrichment is superior to traditional PCR-based Sanger sequencing in terms of both the amount of genomic data that can be recovered and effective cost. Due to their incredibly diverse nature, importance as pollinators, and historical instability with regard to subfamilial and tribal classification, Syrphidae (flower flies or hoverflies) are an ideal candidate for anchored hybrid enrichment-based phylogenetics, especially since recent molecular phylogenies of the syrphids using only a few markers have resulted in highly unresolved topologies. Over 6200 syrphids are currently known and uncovering their phylogeny will help us to understand how these species have diversified, providing insight into an array of ecological processes, from the development of adult mimicry, the origin of adult migration, to pollination patterns and the evolution of larval resource utilization. Results: We present the first use of anchored hybrid enrichment in insect phylogenetics on a dataset containing 30 flower fly species from across all four subfamilies and 11 tribes out of 15. To produce a phylogenetic hypothesis, 559 loci were sampled to produce a final dataset containing 217,702 sites. We recovered a well resolved topology with bootstrap support values that were almost universally >95 %. The subfamily Eristalinae is recovered as paraphyletic, with the strongest support for this hypothesis to date. The ant predators in the Microdontinae are sister to all other syrphids. Syrphinae and Pipizinae are monophyletic and sister to each other. Larval predation on soft-bodied hemipterans evolved only once in this family. Conclusions: Anchored hybrid enrichment was successful in producing a robustly supported phylogenetic hypothesis for the syrphids. Subfamilial reconstruction is concordant with recent phylogenetic hypotheses, but with much higher support values. With the newly designed probe kit this analysis could be rapidly expanded with further sampling, opening the door to more comprehensive analyses targeting problem areas in syrphid phylogenetics and ecology. Peer reviewed
Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4928351Data sources: PubMed CentralFachrepositorium LebenswissenschaftenArticle . 2016License: CC BYData sources: Fachrepositorium LebenswissenschaftenHELDA - Digital Repository of the University of HelsinkiArticle . 2017 . Peer-reviewedData sources: HELDA - Digital Repository of the University of Helsinkiadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 93 citations 93 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!more_vert Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4928351Data sources: PubMed CentralFachrepositorium LebenswissenschaftenArticle . 2016License: CC BYData sources: Fachrepositorium LebenswissenschaftenHELDA - Digital Repository of the University of HelsinkiArticle . 2017 . Peer-reviewedData sources: HELDA - Digital Repository of the University of Helsinkiadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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description Publicationkeyboard_double_arrow_right Article 2016 FinlandPublisher:Springer Science and Business Media LLC Funded by:NSERC, NSF | Beyond Drosophila: Compar..., NSF | I-Corps: Anchored Hybrid ...NSERC ,NSF| Beyond Drosophila: Comparative transcriptomics of schizophoran flies to resolve a rapid radiation ,NSF| I-Corps: Anchored Hybrid EnrichmentAndrew D. Young; Alan R. Lemmon; Jeffrey H. Skevington; Ximo Mengual; Gunilla Ståhls; Menno Reemer; Kurt Jordaens; Scott Kelso; Emily Moriarty Lemmon; Martin Hauser; Marc De Meyer; Bernhard Misof; Brian M. Wiegmann;Background: Anchored hybrid enrichment is a form of next-generation sequencing that uses oligonucleotide probes to target conserved regions of the genome flanked by less conserved regions in order to acquire data useful for phylogenetic inference from a broad range of taxa. Once a probe kit is developed, anchored hybrid enrichment is superior to traditional PCR-based Sanger sequencing in terms of both the amount of genomic data that can be recovered and effective cost. Due to their incredibly diverse nature, importance as pollinators, and historical instability with regard to subfamilial and tribal classification, Syrphidae (flower flies or hoverflies) are an ideal candidate for anchored hybrid enrichment-based phylogenetics, especially since recent molecular phylogenies of the syrphids using only a few markers have resulted in highly unresolved topologies. Over 6200 syrphids are currently known and uncovering their phylogeny will help us to understand how these species have diversified, providing insight into an array of ecological processes, from the development of adult mimicry, the origin of adult migration, to pollination patterns and the evolution of larval resource utilization. Results: We present the first use of anchored hybrid enrichment in insect phylogenetics on a dataset containing 30 flower fly species from across all four subfamilies and 11 tribes out of 15. To produce a phylogenetic hypothesis, 559 loci were sampled to produce a final dataset containing 217,702 sites. We recovered a well resolved topology with bootstrap support values that were almost universally >95 %. The subfamily Eristalinae is recovered as paraphyletic, with the strongest support for this hypothesis to date. The ant predators in the Microdontinae are sister to all other syrphids. Syrphinae and Pipizinae are monophyletic and sister to each other. Larval predation on soft-bodied hemipterans evolved only once in this family. Conclusions: Anchored hybrid enrichment was successful in producing a robustly supported phylogenetic hypothesis for the syrphids. Subfamilial reconstruction is concordant with recent phylogenetic hypotheses, but with much higher support values. With the newly designed probe kit this analysis could be rapidly expanded with further sampling, opening the door to more comprehensive analyses targeting problem areas in syrphid phylogenetics and ecology. Peer reviewed
Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4928351Data sources: PubMed CentralFachrepositorium LebenswissenschaftenArticle . 2016License: CC BYData sources: Fachrepositorium LebenswissenschaftenHELDA - Digital Repository of the University of HelsinkiArticle . 2017 . Peer-reviewedData sources: HELDA - Digital Repository of the University of Helsinkiadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1186/s12862-016-0714-0&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 93 citations 93 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!more_vert Europe PubMed Centra... arrow_drop_down Europe PubMed CentralArticle . 2016Full-Text: http://europepmc.org/articles/PMC4928351Data sources: PubMed CentralFachrepositorium LebenswissenschaftenArticle . 2016License: CC BYData sources: Fachrepositorium LebenswissenschaftenHELDA - Digital Repository of the University of HelsinkiArticle . 2017 . Peer-reviewedData sources: HELDA - Digital Repository of the University of Helsinkiadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1186/s12862-016-0714-0&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu